Objective To construct a patient-centered evaluation index system for the quality of assisted reproduction specialty care and to conduct preliminary validation to provide a basis for the rational evaluation of the quality of assisted reproduction specialty care services and the standardized construction of service content. Methods Based on the Servqual model,through systematic literature search and combining with specialty characteristics,we constructed the first draft of the nursing quality evaluation index system for assisted reproduction specialties. 2 rounds of correspondence with 17 experts from 5 provinces were carried out to evaluate,screen and revise the evaluation index system by applying the Delphi method from July to August 2024,and 40 nurses specializing in assisted reproduction were selected in Jiangsu Province in June 2025 for the preliminary validation of the application of the index system. Results The recovery rates of the questionnaires for the 2 rounds of expert correspondence were 94.44% and 100%,respectively;the expert authority coefficients were 0.927 and 0.908,respectively;the coefficients of variation of the indicators were 0~0.24 and 0~0.18,respectively;the Kendall’s harmonization coefficients were 0.220 and 0.313. After 2 rounds of expert correspondence,the final evaluation index system of assisted reproduction specialty nursing quality was formed,which contained 5 primary indicators,17 secondary indicators,and 41 tertiary indicators. Assisted reproduction specialty nurses were generally satisfied with this evaluation index system,with a satisfaction questionnaire score of (23.75±2.02). Conclusion The quality evaluation index system of assisted reproduction specialty care constructed based on Servqual model covers the key aspects of assisted reproduction care service,with reasonable content setting,standardized construction method,and strong scientificity,reliability,practicality and feasibility.