Objective To develop a follow-up protocol for lymphoma patients undergoing chemotherapy and to evaluate its application effects,aiming to provide a reference for standardizing lymphoma follow-up practice. Methods Guided by the theoretical framework of “interactive patient participation in patient safety”,a follow-up protocol was developed through literature review,semi-structured interviews,expert consultation,and a pilot test. Using consecutive sampling,65 lymphoma patients hospitalized in a tertiary grade A general hospital in Fujian Province from July to November 2023 were recruited. Patients admitted from September to November 2023 were assigned to an experimental group and received the developed follow-up protocol,while those admitted from July to August 2023 were assigned to a control group and received routine follow-up care. The follow-up lasted for 3 chemotherapy cycles. Differences in self-care ability,treatment compliance,anxiety and depression scores,and follow-up satisfaction were compared between the 2 groups. Results 4 patients dropped out during the study. Finally,32 patients in the experimental group and 29 in the control group completed the study. After the follow-up,the experimental group showed significantly higher scores in self-care ability,treatment compliance,and follow-up satisfaction,and significantly lower anxiety and depression scores compared to the control group(all P<0.05). Conclusion The follow-up protocol for lymphoma patients undergoing chemotherapy demonstrates good scientific rigor and feasibility. The implementation of this protocol can improve patients’ self-care ability,enhance their treatment compliance and follow-up satisfaction,and alleviate their anxiety and depression.