Objective To investigate the demand of specialty nurses in China for retraining,and to provide references for the continuous development of specialty nurses. Methods A convenient sampling method was used to survey specialty nurses from all 31 provinces in May-June 2023. A self-designed questionnaire was used as an electronic questionnaire. Results A total of 61,562 valid questionnaires were collected,with 70.0% of specialty nurses believing that it is “very necessary” or “necessary” to carry out retraining for specialty nurses,and 68.4% being “very willing” or “willing” to participate in further training;64.6% chose a five-year cycle for retraining;and 66.5% chose online learning as their preferred mode of learning. In terms of content and evaluation methods for retraining,the top 3 in the retraining content were specialized knowledge(88.9%),clinical practice(74.3%),and nursing research(59.5%). The top 3 in retraining evaluation were specialized knowledge(87.5%),clinical practice ability(72.2%),and teaching guidance ability(44.3%). Conclusion It is feasible and necessary to carry out the retraining for specialty nurses. It is suggested that the training institutions should carry out the retraining in time according to the characteristics of each specialty and the wishes of specialty nurses,so as to promote the knowledge update and ability maintenance of specialty nurses.