Objective A nurse-led standardized process for regional transport of mobile extracorporeal membrane oxygenation(ECMO) patients was constructed and applied,and its application effect was analyzed. Methods Based on the opinions of extracorporeal life support organization ECMO transport guidelines,a nurse-led standardized process for the regional transport of mobile ECMO patients was constructed through literature search,expert consultation and exercise testing. A convenient sampling method was used to continuously select 82 patients who applied mobile ECMO out-of-hospital treatment in the general care unit of a tertiary hospital in Zhejiang Province from August 2020 to July 2022 as the study subjects,including 47 cases admitted in August 2021 to July 2022 as the experimental group,and 35 cases admitted from August 2020 to July 2021 as the control group. The experimental group was implemented by a nurse-led standardized transport process for mobile ECMO patients,and the control group was implemented by a routine mobile ECMO patient transport process,and the differences in material preparation time,departure time of personnel,time from towel laying to transferring,and transport adverse event rate were compared between the 2 groups. Results There were no significant differences in gender,age,and disease type between the 2 groups. Compared with the control group,the material preparation time(10.09±2.96)minutes, the departure time of personnel arrival (12.74±4.61) minutes and the time from towel laying to transferring(31.40±6.44) minutes were significantly shortened, and the incidence of adverse events in the experimental group decreased significantly. The differences were statistically significant(P<0.05). Conclusion The application of the nurse-led standardized process for regional transport of mobile ECMO patients can shorten the time related to transport and reduce the incidence of adverse events during transport,and the nurse-led transport can improve its efficiency and reduce the transport risk of ECMO patients.