Objective To summarize the best evidence of airway obstruction prevention and management in patients undergoing cervical spinal surgery,and to apply evidence-based nursing practice and evaluate the effects. Methods The best evidence for prevention and management of airway obstruction in patients undergoing cervical surgery was summarized using an evidence-based care approach,and an evidence-based practice protocol was developed. The best evidence application strategy was constructed after baseline review and analysis of barriers,and it was implemented in the department of orthopedics in a tertiary hospital in Hubei Province from August 2020 to December 2021. The knowledge level of nurses about airway obstruction and the implementation rate of the reviewed indicators were compared before and after the application of evidence. Results After the application of best evidence, the implementation rate of 19 review indicators out of 23 significantly improved compared with baseline review(P<0.05). The score of nurses’ knowledge level of airway obstruction after cervical spine surgery increased from(48.38±9.40) to (53.49±9.67),with a statistically significant difference(P<0.001). Conclusion The application of the best evidence for prevention and management of airway obstruction after cervical spine surgery can improve nurses’ implementation rate,improve their knowledge level,and ensure patient safety.