Objective To apply the best evidence for out-hospital management of breast milk in hospitalized neonates to clinical practice and to evaluate its effects. Methods We used the method of evidence-based nursing to obtain the best evidence. From September 2021 to February 2022，the evidence was applied to the clinical practice through 4 steps，including evidence acquisition，baseline evaluation，evidence introduction，and after-effect evaluation. The incidence of breast milk-related infection，nurses and maternal women’s cognitive level of out-hospital management about breast milk，the implementation rate of review items about maternal women were compared before and after the application of evidence-based practice. Results We finally included 21 pieces of evidence，with a total of 18 review indicators. The incidence of breast milk-related infection decreased from 5.81% to 0（P=0.026）;the scores of nurses and maternal women on out-hospital management of breastfeeding increased from（70.1±5.4） and（55.8±8.3） to（87.6±5.0） and（74.5±6.2）（t=10.606，P<0.001;t=8.055，P<0.001）;except for items 8，13，and 16，the compliance of out-hospital management review indicators for breastfeeding increased to more than 80%. Conclusion Evidence-based practice can standardize the correct operation of family members’ breast milk collection，storage，and transportation，thereby reducing the risk of breast milk contamination and ensuring the quality and safety of breast milk.
